[Histonet] Slide Staining Variability

[Mike Pence]

We have had some of these same problems and it is always been a nozzle
or mixer problem.  Do you perform regular PM's?  The reaction buffer ph
could also be off, that can cause varied staining.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Annette
Hall
Sent: Thursday, August 09, 2007 8:42 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide Staining Variability


Histonetters,

For those of you who use automated IHC staining systems, has anyone had
issues with part of the slide staining and the rest of the slide not? We
have adopted the policy of placing the control on with the patient
tissue as a more comprehensive check of the system. However, we
occasionally find the control is acceptable but the patient fails to
even counterstain. This has been an intermittent problem on the Ventana
Benchmark for some time, but the frequency has been increasing to almost
daily.

Any thoughts?

Thanks,
Annette J Hall
Histo/Cyto/Micro Supervisor
United Clinical Laboratories
Dubuque, IA 52001

-----Original Message-----
From: Harrison, Sandra C. [mailto:Sandra.Harrison3 <@t> va.gov] 
Sent: Monday, August 06, 2007 3:35 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica autostainer w/integrated coverslipper

Histonetters,
Could anyone tell me if they're using the Leica AutoStainer XL (ST5010)
with the integrated coverslipper (CV5030)? Also, can you tell me if
you've experienced any trouble with it and how long you've been using
it?

Thanks,
Sandy
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