[Histonet] Slide Staining Variability

[Annette Hall]

Histonetters,

For those of you who use automated IHC staining systems, has anyone had
issues with part of the slide staining and the rest of the slide not? We
have adopted the policy of placing the control on with the patient tissue as
a more comprehensive check of the system. However, we occasionally find the
control is acceptable but the patient fails to even counterstain. This has
been an intermittent problem on the Ventana Benchmark for some time, but the
frequency has been increasing to almost daily.

Any thoughts?

Thanks,
Annette J Hall
Histo/Cyto/Micro Supervisor
United Clinical Laboratories
Dubuque, IA 52001

-----Original Message-----
From: Harrison, Sandra C. [mailto:Sandra.Harrison3 <@t> va.gov] 
Sent: Monday, August 06, 2007 3:35 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica autostainer w/integrated coverslipper

Histonetters,
Could anyone tell me if they're using the Leica AutoStainer XL (ST5010)
with the integrated coverslipper (CV5030)?
Also, can you tell me if you've experienced any trouble with it and how
long you've been using it?

Thanks,
Sandy
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