[Histonet] question about cresyl violet

Kemlo Rogerson Kemlo.Rogerson <@t> waht.swest.nhs.uk
Mon Aug 6 09:23:50 CDT 2007


You are trying to demonstrate Nissl substance?

Add 0.4 ml 10% acetic acid to 40 mls 0.1% cresyl echt violet in dist
water, warm (35 to 40 degrees C). Stain sections 6 min, differentiate in
95% alcohol, dehydrate in two changes of absolute alcohol, clear in
xylene and mount in synthetic resin.

Kemlo Rogerson
Pathology Manager
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