[Histonet] trichrome problems

sheila adey sheila_adey <@t> hotmail.com
Fri Aug 3 20:49:44 CDT 2007

We put our slides in an 74 degree oven for 10 minutes and then it gets the 
12 min bake on the stainer going to water. This has improved our problems 
with the sections lifting off.

Sheila Adey HT MLT
Port Huron Hospital

>From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
>To: "Derek Papalegis" 
><derek.papalegis <@t> tufts.edu>,<histonet <@t> lists.utsouthwestern.edu>
>Subject: RE: [Histonet] trichrome problems
>Date: Thu, 2 Aug 2007 10:55:41 -0400
>We routinely leave our slides in a 60 degree oven overnight before 
>Peggy Sherwood
>Lab Associate, Photopathology
>Wellman Center for Photomedicine (W224)
>Massachusetts General Hospital
>55 Fruit Street
>Boston, MA 02114-2696
>617-724-4839 (voice mail)
>617-726-6983 (lab)
>617-726-1206 (fax)
>msherwood <@t> partners.org
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Derek
>Sent: Thursday, August 02, 2007 10:28 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] trichrome problems
>Hi Everyone,
>I am having trouble with the tissue staying on the slide when doing a
>trichrome stain. I currently use the Richard Allen super up-rite slides.
>I found that the tissue came off the slide after I put it in the heated
>bouin's solution for an hour. I tried to leave it in room temp bouin's
>overnight instead of using it heated and I found that some tissue has
>stayed on but some has still fallen off. I have tried both leaving
>slides out to dry overnight as well as cutting and staining on the same
>day. Does anyone have any ideas of how I can solve this problem?
>Derek Papalegis HT (ASCP)
>Division of Laboratory Animal Medicine
>Tufts University
>136 Harrison Avenue
>Boston, MA 02111
>phone: 617 636-2971
>fax: 617 636-8354
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