[Histonet] trichrome problems

[Derek Papalegis]

Hi Everyone,
I am having trouble with the tissue staying on the slide when doing a 
trichrome stain. I currently use the Richard Allen super up-rite slides. 
I found that the tissue came off the slide after I put it in the heated 
bouin's solution for an hour. I tried to leave it in room temp bouin's 
overnight instead of using it heated and I found that some tissue has 
stayed on but some has still fallen off. I have tried both leaving 
slides out to dry overnight as well as cutting and staining on the same 
day. Does anyone have any ideas of how I can solve this problem?

Thanks,
Derek

-- 
Derek Papalegis HT (ASCP)
Histotechnician
Division of Laboratory Animal Medicine
Tufts University 
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354