[Histonet] To Albert C re: Fatty tissue blowing up on waterbath

[Larry Woody]

Put the section on the slide dry and then lower the slide with the tissue on it into the water bath as evenly as you can. When the water comes onto the slide and under the tissue raise it out of the water immediately. Good Luck!

Mike Pence <mpence <@t> grhs.net> wrote:  What does this do to tissue you are going to use for IHC?
Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nick and
Amanda
Sent: Thursday, August 02, 2007 2:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] To Albert C re: Fatty tissue blowing up on waterbath


Albert, 

I was shown a simple trick years ago and have introduced it to many
people since. Simply one single drop of household detergent (not
budget, it doesn't work!) onto the waterbath changes the surface tension
of the water and holds your fatty sections together..

Hope this is helpful! we use it all the time..
Manda of Wellington,NZ _______________________________________________
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