[Histonet] Re: Finding Lymph Nodes After Fixation

[Wiese, Jason VHAROS]

I disagree.  I use Presto-Fixe II from spectra-tint for fat and lymph
node.  Even after formalin fixation, you can throw your pericolonic fat,
etc.. in it, and it will turn the lymph nodes white...

Jason Wiese, BS, HT(ASCP)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
rsrichmond <@t> aol.com
Sent: Tuesday, April 24, 2007 10:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Finding Lymph Nodes After Fixation

Wanda Smith asks:

>>One of my pathologists asked me to ask a question - Has anyone had 
success in finding colonic lymph nodes in fat AFTER
fixation with 10% neutral buffered formalin? This pathologist has tried 
Dissect-aid and other solutions that seem to work on fresh fat, but he 
has had no luck trying this after the fat has been in formalin.<<

No, this doesn't work. Though often when the specimen arrives in 
formalin the fat has been so little penetrated by formalin that using a 
disclosing fixative is still worthwhile.

Any other specialty, you'd be able to make arrangements with the 
operating room staff to solve this problem. In pathology, the 
red-haired stepchild again. And the clinical stakes are high here - one 
positive lymph node, however small, requires chemotherapy.

Bob Richmond
Samurai Pathologist
Knoxville TN
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