[Histonet] Re: Finding Lymph Nodes After Fixation
Wiese, Jason VHAROS
Jason.Wiese <@t> va.gov
Tue Apr 24 13:38:47 CDT 2007
I disagree. I use Presto-Fixe II from spectra-tint for fat and lymph
node. Even after formalin fixation, you can throw your pericolonic fat,
etc.. in it, and it will turn the lymph nodes white...
Jason Wiese, BS, HT(ASCP)
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
rsrichmond <@t> aol.com
Sent: Tuesday, April 24, 2007 10:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Finding Lymph Nodes After Fixation
Wanda Smith asks:
>>One of my pathologists asked me to ask a question - Has anyone had
success in finding colonic lymph nodes in fat AFTER
fixation with 10% neutral buffered formalin? This pathologist has tried
Dissect-aid and other solutions that seem to work on fresh fat, but he
has had no luck trying this after the fat has been in formalin.<<
No, this doesn't work. Though often when the specimen arrives in
formalin the fat has been so little penetrated by formalin that using a
disclosing fixative is still worthwhile.
Any other specialty, you'd be able to make arrangements with the
operating room staff to solve this problem. In pathology, the
red-haired stepchild again. And the clinical stakes are high here - one
positive lymph node, however small, requires chemotherapy.
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