[Histonet] Re: Finding Lymph Nodes After Fixation

[Weems, Joyce]

We use Clark's fixative = Carnoy's without chloroform. It works well
even after the specimen has been in formalin. 


Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
rsrichmond <@t> aol.com
Sent: Tuesday, April 24, 2007 1:55 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Finding Lymph Nodes After Fixation

Wanda Smith asks:

>>One of my pathologists asked me to ask a question - Has anyone had
success in finding colonic lymph nodes in fat AFTER fixation with 10%
neutral buffered formalin? This pathologist has tried Dissect-aid and
other solutions that seem to work on fresh fat, but he has had no luck
trying this after the fat has been in formalin.<<

No, this doesn't work. Though often when the specimen arrives in
formalin the fat has been so little penetrated by formalin that using a
disclosing fixative is still worthwhile.

Any other specialty, you'd be able to make arrangements with the
operating room staff to solve this problem. In pathology, the red-haired
stepchild again. And the clinical stakes are high here - one positive
lymph node, however small, requires chemotherapy.

Bob Richmond
Samurai Pathologist
Knoxville TN
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