[Histonet] Zinc Formalin and Iron stains

Patsy Ruegg pruegg <@t> ihctech.net
Wed Apr 18 16:00:14 CDT 2007

I have been using commercially made zinc formalin instead of formalin for
the past 10+ years, I have not ever seen it make my tissues brittle or
precipitate in any of the reagents on the tissue processor.  As for
retaining iron, there are other issues to consider besides just the
fixative.  Even alcohol dehydration can remove iron, (anything acidic), when
I was in hematopathology we always did the iron stain on smears or GMA
processed bone marrows without decal.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Sent: Tuesday, April 17, 2007 12:42 AM
To: Liz Chlipala; Gayle Callis; Tarango, Mark;
Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Zinc Formalin and Iron stains

Zinc also mordents haematoxylin resulting in a very intense nuclear
stain; makes tissue brittle too.

Kemlo Rogerson
Pathology Manager
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