[Histonet] Zinc Formalin fixation

Gayle Callis gcallis <@t> montana.edu
Tue Apr 17 10:08:37 CDT 2007

We never had a problem with tissue being brittle after fixation with the 
commercial Anatech zinc formalin (buffered) although I have heard others 
complain about this along with Liz's comments about precipitation problems, 
maybe in house prepared zinc formalin?  However, the zinc TRIS buffer 
fixative, that is FORMALIN FREE, for CD marker immunohistochemistry 
(Beckstead, J Histochem Cytochem) made tissues terrible brittle.

At 12:41 AM 4/17/2007, you wrote:
>Zinc also mordents haematoxylin resulting in a very intense nuclear
>stain; makes tissue brittle too.
>Kemlo Rogerson
>Pathology Manager
>DD   01934 647057 or extension 3311
>Mob 07749 754194; Pager 07659 597107;

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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