[Histonet] validation of IHC slides for Benchmark Stainer

[Kellar, Eric C]

It's best to keep the original slides from your validation indefinitely along with the original signed validation procedure documentation. I keep mine in a binder with original Benchmark signed validation document and slide sleeve holding original slides (some remounted after being broken since 2003) used to validate each antibody. If we are to keep blocks and slides for 10 years, why would the original documentation of antibody validation and the slides be any different? Keep them for future reference. Future CAP inspectors will be glad you did, and it will save you and your colleagues from revalidating those antibodies again.

Eric C. Kellar
Quest Diagnostics 
Histology Laboratory Supervisor
South Florida



 -----Original Message-----
From: 	histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]  On Behalf Of Cindy Robinson
Sent:	Wednesday, April 11, 2007 10:25 AM
To:	histonet <@t> lists.utsouthwestern.edu; Tiffany Price
Subject:	Re: [Histonet] validation of IHC slides for Benchmark Stainer

We kept all of our slides from our recent XT install in November and were just CAP inspected yesterday.  I did document on a spreadsheet the final protocols.  As we passed our inspection with no hits I think I can discard the slides now and just keep paperwork.

Cindi Robinson, HT (ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049

>>> "Price, Tiffany" <Tiffany.Price <@t> thomaswv.org> 4/11/2007 9:06 AM >>>
Could someone tell me if I need to keep all of the slides that I stained
to optimize my IHC protocols, the slide for the final protocol I keep,
or is a printed log signed off by the Pathologist sufficient for
documentation?
Thanks
Tiffany
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