[Histonet] bouins fixed mouse tissue

[Geoff McAuliffe]

Shortening the fixation in Bouin's will NOT solve the problem and it may 
make it worse. Tissue can be left in Bouin's for a week or more without 
harm.

Geoff

Akemi Allison-Tacha wrote:

>Helen,
>As you probably know, mouse tissue is much more
>delicate than human tissue.  If you have control over
>the fixation process, I would cut down the initial
>fixation time in Bouin's solution.  Perhaps, try 6-12
>hours.  
>
>For the tissue that you have already processed, soak
>in a tween 80 and water mixture of 1:10 for a minimum
>of 10 minutes, followed by a water wash to remove
>tween, then soak on a ice tray.  This works
>beautifully for over-processed tissue, skin, uterus,
>ect.
>
>PS: Tween 20 can be substituted or a similar
>surfactant.
>
>Good Luck,
>Akemi Allison-Tacha BS, HT (ASCP) HTL
>President
>Phoenix Lab Consulting & Staffing
>Specializing in Histology, SS, IHC, & Microarray
>Madison, WI
>Tele: 	(925) 788-0900
>E-Mail:	akemiat3377 <@t> yahoo.com
>
>--- Andrea Grantham <algranth <@t> u.arizona.edu> wrote:
>
>  
>
>>helen,
>>What kind of tissue are you trying to cut?
>>I frequently get mouse ovaries fixed for 24 hrs in
>>Bouins and transfered to 
>>70% ETOH. They usually cut fine but in your case my
>>suggestion is SOAK, 
>>SOAK, SOAK in icy water.
>>Not long ago someone here suggested putting a little
>>glycerin in the 
>>soaking water - you can find the actual posts on the
>>archives - but I tried 
>>it and was delighted with the results.
>>
>>Andi Grantham
>>
>>
>>At 02:07 PM 4/9/2007 -0400, Helen E Johnson wrote:
>>
>>    
>>
>>>Dear Histonetters,
>>>           Has anyone experienced chatter artifact
>>>      
>>>
>>while sectioning Bouins
>>    
>>
>>>fixed mouse tissue?  The tissue is fixed in Bouins
>>>      
>>>
>>fixative for 24 hrs. and
>>    
>>
>>>transferred to 70% EtOH for rinsing.  The tissues
>>>      
>>>
>>are processed 15 min. at
>>    
>>
>>>each solution station.
>>>Helen Johnson    (hej01 <@t> health.state.ny.us.)
>>>
>>>
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>>>Histonet <@t> lists.utsouthwestern.edu
>>>      
>>>
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>    
>>
>.....................................................................
>  
>
>>: Andrea Grantham, HT(ASCP)     Dept. of Cell
>>Biology & Anatomy     :
>>: Sr. Research Specialist       University of
>>Arizona               :
>>: (office:  AHSC 4212)          P.O. Box 245044     
>>               :
>>: (voice:  520-626-4415)        Tucson, AZ 
>>85724-5044    USA       :
>>: (FAX:  520-626-2097)          (email: 
>>algranth <@t> u.arizona.edu)       :
>>
>>    
>>
>:...................................................................:
>  
>
>>          
>>http://www.cba.arizona.edu/histology-lab.html
>>
>>
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