[Histonet] bouins fixed mouse tissue

[Akemi Allison-Tacha]

Helen,
As you probably know, mouse tissue is much more
delicate than human tissue.  If you have control over
the fixation process, I would cut down the initial
fixation time in Bouin's solution.  Perhaps, try 6-12
hours.  

For the tissue that you have already processed, soak
in a tween 80 and water mixture of 1:10 for a minimum
of 10 minutes, followed by a water wash to remove
tween, then soak on a ice tray.  This works
beautifully for over-processed tissue, skin, uterus,
ect.

PS: Tween 20 can be substituted or a similar
surfactant.

Good Luck,
Akemi Allison-Tacha BS, HT (ASCP) HTL
President
Phoenix Lab Consulting & Staffing
Specializing in Histology, SS, IHC, & Microarray
Madison, WI
Tele: 	(925) 788-0900
E-Mail:	akemiat3377 <@t> yahoo.com

--- Andrea Grantham <algranth <@t> u.arizona.edu> wrote:

> helen,
> What kind of tissue are you trying to cut?
> I frequently get mouse ovaries fixed for 24 hrs in
> Bouins and transfered to 
> 70% ETOH. They usually cut fine but in your case my
> suggestion is SOAK, 
> SOAK, SOAK in icy water.
> Not long ago someone here suggested putting a little
> glycerin in the 
> soaking water - you can find the actual posts on the
> archives - but I tried 
> it and was delighted with the results.
> 
> Andi Grantham
> 
> 
> At 02:07 PM 4/9/2007 -0400, Helen E Johnson wrote:
> 
> >Dear Histonetters,
> >            Has anyone experienced chatter artifact
> while sectioning Bouins
> >fixed mouse tissue?  The tissue is fixed in Bouins
> fixative for 24 hrs. and
> >transferred to 70% EtOH for rinsing.  The tissues
> are processed 15 min. at
> >each solution station.
> >Helen Johnson    (hej01 <@t> health.state.ny.us.)
> >
> >
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>
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
>
.....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell
> Biology & Anatomy     :
> : Sr. Research Specialist       University of
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> algranth <@t> u.arizona.edu)       :
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