[Histonet] bouins fixed mouse tissue

[Andrea Grantham]

helen,
What kind of tissue are you trying to cut?
I frequently get mouse ovaries fixed for 24 hrs in Bouins and transfered to 
70% ETOH. They usually cut fine but in your case my suggestion is SOAK, 
SOAK, SOAK in icy water.
Not long ago someone here suggested putting a little glycerin in the 
soaking water - you can find the actual posts on the archives - but I tried 
it and was delighted with the results.

Andi Grantham


At 02:07 PM 4/9/2007 -0400, Helen E Johnson wrote:

>Dear Histonetters,
>            Has anyone experienced chatter artifact while sectioning Bouins
>fixed mouse tissue?  The tissue is fixed in Bouins fixative for 24 hrs. and
>transferred to 70% EtOH for rinsing.  The tissues are processed 15 min. at
>each solution station.
>Helen Johnson    (hej01 <@t> health.state.ny.us.)
>
>
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: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
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