[Histonet] X-gal staining of adult mouse spinal cord
bs8asg <@t> bath.ac.uk
Sat Nov 18 05:34:35 CST 2006
Could anyone provide me with a protocol for the sectioning and x-gal
staining of adult spinal cord? I presume cryosections are my best
bet....does the tissue require decalcification and if so how is that
achieved? Can i get away without fixation? It would ensure i don't
compromise B-gal activity.
Apologies for the barrage of questions. Any help is appreciated.
University of Bath
Centre for Regenerative Medicine
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