[Histonet] X-gal staining of adult mouse spinal cord

[al garfield]

Dear People,

Could anyone provide me with a protocol for the sectioning and x-gal 
staining of adult spinal cord? I presume cryosections are my best 
bet....does the tissue require decalcification and if so how is that 
achieved? Can i get away without fixation? It would ensure i don't 
compromise B-gal activity.

Apologies for the barrage of questions. Any help is appreciated.
cheers
al garfield

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University of Bath
Centre for Regenerative Medicine
UK