[Histonet] X-gal staining of adult mouse spinal cord

Keller, Charles kellerc2 <@t> uthscsa.edu
Tue Nov 28 10:51:58 CST 2006

The last few pages are the adult x-gal staining method.  Hope it works
for you.  Sincerely, Charles

Charles Keller, MD
Assistant Professor, Department of Cellular & Structural Biology
Adjunct Assistant Professor, Department of Pediatrics
Children's Cancer Research Institute
The University of Texas Health Science Center
7703 Floyd Curl Drive, Mail Code 7784
San Antonio, TX 78229-3900
210-562-9062 [office]
210-562-9014 [fax]
http://ccri.uthscsa.edu or
kellerc2 <@t> uthscsa.edu
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of al
Sent: Saturday, November 18, 2006 5:35 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] X-gal staining of adult mouse spinal cord

Dear People,

Could anyone provide me with a protocol for the sectioning and x-gal 
staining of adult spinal cord? I presume cryosections are my best 
bet....does the tissue require decalcification and if so how is that 
achieved? Can i get away without fixation? It would ensure i don't 
compromise B-gal activity.

Apologies for the barrage of questions. Any help is appreciated.
al garfield

University of Bath
Centre for Regenerative Medicine

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