[Histonet] Re: TUNEL methods

[Ales Kladnik]

we used the Roche kit for TUNEL assay on paraffin embedded plant tissue 
sections. for the actual tunel reaction you don't need additional 
buffers, just mix the Labeling and Enzyme solutions. for washing steps 
we used PBS.
i have a pdf-version of our protocol, so please email me if you're 
interested.

-- 
Aleš Kladnik
University of Ljubljana, Biotechnical Faculty, Department of Biology
Večna pot 111, SI-1000 Ljubljana, Slovenia
tel: +386 1 4233388, fax: +386 1 2573390
url: http://botanika.biologija.org/
skype: fridjo, msn: aleskladnik <@t> hotmail.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Muskett
David
Sent: Friday, November 17, 2006 4:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] TUNEL methods


Dear All

We are starting a project using tunel and the kit insert leaflet we have
from Roche is not very clear. Does anyone have a tunel method (with
buffers required) that they would be willing to share.

Thanks

David