[Histonet] endothelial cell specific lectin staining

[Gayle Callis]

Dear Mark in very snowy Vancouver Canada,

There is a lectin specific for endothelial cells.  Go to Vector and check 
out one of their brochure on latest products with info about the  tomato 
lectin.  You can perfuse the animal OR use it as a direct conjugate on 
tissue sections for fluorescent work.    Vector may even have an anti 
tomato lectin antibody available or lectin conjugated to biotin for IHC 
work.  This would eliminate rabbit on rabbit work entirely or you could 
detect lectin-FITC with an antiFITC.

I just ran across a coverphoto of this on BioTechniques, a freebie magazine 
39(4)October 2005 and the lectin was applied to rat retinal tissue and it 
also works in murine tissue.  If you can't locate the info on Vector 
website, I have a citation for this in Am J of Pathology.

Good luck





Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)