[Histonet] endothelial cell specific lectin staining- perfusion

[Adi Sabag]

Dear Gayle, 
Do you have a good protocol for lactin pefusion?
What about Dextran?

Thanks- 

Adi

Quoting Gayle Callis <gcallis <@t> montana.edu>:

> Dear Mark in very snowy Vancouver Canada,
> 
> There is a lectin specific for endothelial cells.  Go to Vector and check 
> out one of their brochure on latest products with info about the  tomato 
> lectin.  You can perfuse the animal OR use it as a direct conjugate on 
> tissue sections for fluorescent work.    Vector may even have an anti 
> tomato lectin antibody available or lectin conjugated to biotin for IHC 
> work.  This would eliminate rabbit on rabbit work entirely or you could 
> detect lectin-FITC with an antiFITC.
> 
> I just ran across a coverphoto of this on BioTechniques, a freebie magazine 
> 39(4)October 2005 and the lectin was applied to rat retinal tissue and it 
> also works in murine tissue.  If you can't locate the info on Vector 
> website, I have a citation for this in Am J of Pathology.
> 
> Good luck
> 
> 
> 
> 
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
> 
> 
> 
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-- 
Adi Sabag
Gera Neufeld's laboratory
Medicine Faculty, Technion
04-8295425