[Histonet] endothelial cell specific lectin staining- perfusion

Adi Sabag adisabag <@t> techunix.technion.ac.il
Tue Nov 28 03:11:04 CST 2006

Dear Gayle, 
Do you have a good protocol for lactin pefusion?
What about Dextran?



Quoting Gayle Callis <gcallis <@t> montana.edu>:

> Dear Mark in very snowy Vancouver Canada,
> There is a lectin specific for endothelial cells.  Go to Vector and check 
> out one of their brochure on latest products with info about the  tomato 
> lectin.  You can perfuse the animal OR use it as a direct conjugate on 
> tissue sections for fluorescent work.    Vector may even have an anti 
> tomato lectin antibody available or lectin conjugated to biotin for IHC 
> work.  This would eliminate rabbit on rabbit work entirely or you could 
> detect lectin-FITC with an antiFITC.
> I just ran across a coverphoto of this on BioTechniques, a freebie magazine 
> 39(4)October 2005 and the lectin was applied to rat retinal tissue and it 
> also works in murine tissue.  If you can't locate the info on Vector 
> website, I have a citation for this in Am J of Pathology.
> Good luck
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Adi Sabag
Gera Neufeld's laboratory
Medicine Faculty, Technion

More information about the Histonet mailing list