[Histonet] endothelial cell specific lectin staining- perfusion
adisabag <@t> techunix.technion.ac.il
Tue Nov 28 03:11:04 CST 2006
Do you have a good protocol for lactin pefusion?
What about Dextran?
Quoting Gayle Callis <gcallis <@t> montana.edu>:
> Dear Mark in very snowy Vancouver Canada,
> There is a lectin specific for endothelial cells. Go to Vector and check
> out one of their brochure on latest products with info about the tomato
> lectin. You can perfuse the animal OR use it as a direct conjugate on
> tissue sections for fluorescent work. Vector may even have an anti
> tomato lectin antibody available or lectin conjugated to biotin for IHC
> work. This would eliminate rabbit on rabbit work entirely or you could
> detect lectin-FITC with an antiFITC.
> I just ran across a coverphoto of this on BioTechniques, a freebie magazine
> 39(4)October 2005 and the lectin was applied to rat retinal tissue and it
> also works in murine tissue. If you can't locate the info on Vector
> website, I have a citation for this in Am J of Pathology.
> Good luck
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
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