[Histonet] macrophages/microglia in FFPE tissue
zacharyweil <@t> yahoo.com
Mon Nov 27 15:43:26 CST 2006
Hello, I am having a very difficult time labelling microglia/macrophages in FFPE tissue. I have tried a couple of OX-42 (Serotec and Chemicon), MHC-II, several lectins and ED-1 (also serotec) and I am consistently getting no positive staining. This tissue works well for other antigens and I have tried a variety of proteolytic antigen retrieval steps to try and get at these antigens. I know that cell surface markers don't survive paraffin embedding to well but I didnt think the lectins or the ED-1 should be that affected by that. If anyone can suggest anything I would really appreciate it. There are only a couple published papers on hamster tissue (not my hamster species) and I've tried their protocols with no success.
Departments of Psychology and Neuroscience
The Ohio State University
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