[Histonet] macrophages/microglia in FFPE tissue

[Zach Weil]

Hello,  I am having a very difficult time labelling microglia/macrophages in FFPE tissue.  I have tried a couple of OX-42 (Serotec and Chemicon), MHC-II, several lectins and ED-1 (also serotec) and I am consistently getting no positive staining.  This tissue works well for other antigens and I have tried a variety of proteolytic antigen retrieval steps to try and get at these antigens.  I know that cell surface markers don't survive paraffin embedding to well but I didnt think the lectins or the ED-1 should be that affected by that.   If anyone can suggest anything I would really appreciate it.  There are only a couple published papers on hamster tissue (not my hamster species) and I've tried their protocols with no success.  
   
  Thanks,
  Zach
   
  Zachary Weil
  Departments of Psychology and Neuroscience
  The Ohio State University
   

 
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