[Histonet] free floating sections

[Atoska S. Gentry]

Hello, will someone please share with me your procedure for 
collecting & storing free floating frozen sections? This will be a 
first for me. Info gathered from histonet archives indicates the 
sections can be placed into cell culture plates with wells containing 
distilled water.  Is distilled water required or will filtered 
deionized water be sufficient or better yet is it o.k. to place 
phosphate buffer into the wells? Also, please what's the maximum 
amount of time these free floating sections can be held at 4C in the 
culture plates before staining or whatever? I will be sectioning 
these sections for a researcher from main campus and I need to inform 
him. Thanks so much. Atoska