[Histonet] whole villi

[Jackie M O'Connor]

I process mouse GI routinely looking for Caspase 3 in the villi  - We cut 
short lengths of small intestine, open it, and place it (lumen up)  on 
bilbous paper which has been dipped in fixative. The segments stay on the 
paper all the way through paraffin processing.  To embed, I remove the 
segments off the paper, and easily embed multiple segments on edge in one 
block.   The villi remain undamaged.
Jackie O'



"Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/26/2006 10:36 AM

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<histonet <@t> lists.utsouthwestern.edu>
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RE: [Histonet] whole villi






Renee
Do you need to embed and cut them as a circle?
If not I would suggest that you slit along the length and then place the
connective tissue (or muscle) side down on a piece of card. The pieces
should stick to the card. Then fix, remove from card and process as
normal. Once they are fixed they should remain flat.
If you have really big pieces can pin these to a piece of cork and float
tissue side down in the fixative - pin using stainless steel pins,
plastic pins or hedgehog quills.
You will always have trouble in getting sections with entire lengths of
villi in the jejunum because of the long length of these villi in this
region.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Till,
Renee
Sent: Friday, May 26, 2006 10:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] whole villi

I sometimes work with rat and mouse gi tissues, and as you might expect
one of the most time consuming things is getting the tissues embedded
good. The colon has not been as much of a problem, but lately we are
doing more with the jejunum and getting whole villi is enough to make me
scream. It seems to me that one of my main problems is that sometime
between the collection of the tissues (which is not done by me) and when
I embed them, the tissues often curl up and it is hard to get a good
straight piece to embed. We embed them on end? (so when cut it makes a
little O). Any ideas on how to keep them straight during the processing?
Or maybe if there is something else that I am not thinking of that could
be a problem. Thanks.

 

Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72002

Office (501)364-2785

Fax (501)364-3161

 


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