[Histonet] Protocols for estimating the swelling of tissue?

[Ed Boyden]

Many procedures (fixation, immersion in sucrose, etc.) cause swelling
of tissue, versus the intact, original tissue.  Does anyone know of
either:
 1. ways to predict the amount of swelling that occurs with a specific
protocol, or
 2. a protcol that would allow swelling of the tissue by a predictable amount?

I would like to be able to compare samples done at different times,
and stored in different ways.

If not possible, I would like to be able to prepare new samples so
that their swelling will match my old estimates, as much as possible,
or at least that I can calculate the swelling of the new samples.

Thanks so much for your help!

Best,
Ed