[Histonet] Protocols for estimating the swelling of tissue?

[Kemlo Rogerson]

I thought, and I stand to be corrected, that most fixatives caused shrinkage
of tissue. I assumed that to be due to the fixation, condensation, cross
linking of proteins or the eradication of water molecules. Hypertonic
sucrose would also cause shrinkage too as it would exert a negative osmotic
pressure on the 'more dilute' tissue, wouldn't it or have I got my pressures
all wrong.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 

 

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-----Original Message-----
From: Ed Boyden [mailto:eboyden3 <@t> gmail.com] 
Sent: Thursday, May 04, 2006 12:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Protocols for estimating the swelling of tissue?

Many procedures (fixation, immersion in sucrose, etc.) cause swelling
of tissue, versus the intact, original tissue.  Does anyone know of
either:
 1. ways to predict the amount of swelling that occurs with a specific
protocol, or
 2. a protcol that would allow swelling of the tissue by a predictable
amount?

I would like to be able to compare samples done at different times,
and stored in different ways.

If not possible, I would like to be able to prepare new samples so
that their swelling will match my old estimates, as much as possible,
or at least that I can calculate the swelling of the new samples.

Thanks so much for your help!

Best,
Ed

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