[Histonet] RE: Anyone use glass knifes anymore?

Thu May 4 01:51:49 CDT 2006

In  BIll Bryson's "A brief history of nearly everything" he states
that although glass is in fact a supercooled liquid, the amount of
change in sheets of glass, even after hundreds of years is so small as
to be unmeasurable by normal means. I'd lay my bets on the knife
breaker

On 5/3/06, gayle brosnanwatters <gayle.brosnanwatters <@t> sru.edu> wrote:
> I use glass knives (triangles) that I make from 1/2 inch glass on an
> ancient knife maker that I play heck keeping operating. I, too, am
> frustrated by how hard it is to make decent knives.  I section plastic
> embedded mouse brains at between 1 and 3 microns and do light
> microscopy.  Even when my knives are working well, I can expect to use
> two or three per mouse brain, in order to get the sections I want.  My
> glass pieces are not the long strips of 1/4 inch pieces (although I have
> used them), but shorter rectangles.  I think if you can get your knife
> maker working well, it can make a difference.  I just had mine sent off
> to Energy Beam Sciences, and they got it working, but then they shipped
> it back to me and I really think that the shipper dropped it on the way,
> because it has not been working well since.  I understand your
> frustration, although I don't have an answer.  I wondered if the fact
> that my glass is old would make a difference.  I know just enough
> chemistry to remember that glass is a liquid that does change form as it
> ages, and this glass was given to me and was old then.  Does anyone know
> if that would affect it?
> Gayle L. Brosnan-Watters, PhD
> Assistant Professor
> Dept of Psychology
> Treasurer, Faculty for Undergraduate Neuroscience
> 226 Vincent Science Hall
> Slippery Rock University
> Slippery Rock, PA 16057
> gayle.brosnanwatters <@t> sru.edu
> 724-738-2529 - office
> 724-738-4807 - fax
>
> >  I make and use glass knifes for plastic sectioning
> > at 3 microns. I usually have to go through a whole
> > glass strip to get one good knife. Does anyone make
> > them anymore and have the same problem?
> >
> >
> > ~Stephanie Brusig
> >
> > Weyerhaeuser Company
> > Propagation of High Value Trees
> > 32901 Weyerhaeuser Way S
> > WTC-1B10
> > Federal Way, WA 98001
> > 253.924.6518
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> > On Behalf Of
> > histonet-request <@t> lists.utsouthwestern.edu
> > Sent: Wednesday, May 03, 2006 10:10 AM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: Histonet Digest, Vol 30, Issue 3
> >
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> >
> > Today's Topics:
> >
> >    1. CONTROL BLOCKS NEEDED (Nymeyer, Heather)
> >    2. Large sections (Patsy Ruegg)
> >    3. Autostainer tubes  (Patti Loykasek)
> >    4. NSH Active Members (Carrie Diamond)
> >    5. Question (Nita Searcy)
> >    6. Re: Question (Victoria Baker)
> >    7. Re: Large sections (Gayle Callis)
> >    8. RE: Question (Allen, Rhonda)
> >    9. RE: Question (Bartlett, Jeanine (CDC/NCID/VR))
> >   10. Re: Autostainer tubes (Joanne Mauger)
> >   11. Re: POC for Genetic Studies
> > (histology <@t> gradymem.org)
> >   12. antibodies to eIF2 alpha and PPAR gamma (Sarka
> > Lhotak)
> >   13. IHC on frozens (Sarka Lhotak)
> >   14. Fwd: [Histonet] CD Abs (Linresearch <@t> aol.com)
> >   15. gallocyanin stain for nissel  (Elizabeth
> > Chlipala)
> >   16. RANK, ER and PR in mouse (Randolph-Habecker,
> > Julie)
> >   17. NADH staining (Yak-Nam Wang)
> >   18. Re: gallocyanine stain for Nissl (John
> > Kiernan)
> >   19. Inflamatory markers (CRAIG BARLOW)
> >   20. Re- processing fatty tissues (Malam
> > Jacqueline)
> >   21. The end of an era (McGovern, Kevin)
> >   22. Mycobacterium bovis (BCG) antibody (Martha
> > Ward)
> >   23. Glycerol cryoprotected tissue and cryostat
> > sectioning
> >       (Kathie A Berghorn)
> >   24. RE: IHC on frozens (Guillermo Palao)
> >   25. Save These Dates For The Pennsylvania State
> > Histology     Meeting
> >       (Pamela Marcum)
> >   26. RE: Mycobacterium bovis (BCG) antibody
> > (Elizabeth Chlipala)
> >
> >
> >
> ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Tue, 2 May 2006 10:03:23 -0700
> > From: "Nymeyer, Heather"
> > <Heather.Nymeyer <@t> interiorhealth.ca>
> > Subject: [Histonet] CONTROL BLOCKS NEEDED
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >
> >
> <463911DE3F9F8D4AA3EC67632386BC62D1509E <@t> dc1serv78.interiorhealth.ca>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> > We are in need of the following control blocks
> >
> >             - Leprosy bacilli
> >
> >             - Spirochete
> >
> >
> >
> > Your assistance is appreciated and thank you in
> > advance.
> >
> >
> >
> > Heather D. Nymeyer, RT, CEBT
> >
> > Charge Technologist, Anatomic Pathology
> >
> > Royal Inland Hospital,
> >
> > Kamloops, BC.
> >
> > 250-314-2664
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Tue, 2 May 2006 10:52:50 -0600
> > From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> > Subject: [Histonet] Large sections
> > To: <histonet <@t> pathology.swmed.edu>
> > Message-ID:
> > <200605021652.k42Gqoog014137 <@t> chip.viawest.net>
> > Content-Type: text/plain;     charset="US-ASCII"
> >
> > Can anyone suggest a resource for getting frozen
> > sections of Elk Larynx?
> >
> > One of my clients asks:
> > "I study elk sound production. As one question we
> > investigate the anatomical structure of the larynx.
> > For this purpose I need cross sections of several
> > larynges (approx. 15 larynges). Cross sections at
> > three positions are necessary for each larynx,. The
> > larynges are most likely calcified at varying
> > degrees. I have stored the larynges frozen in saline
> > solution."
> >
> > Patsy Ruegg, HT(ASCP)QIHC
> > IHCtech, LLC
> > Fitzsimmons BioScience Park
> > 12635 Montview Blvd. Suite 215
> > Aurora, CO 80010
> > P-720-859-4060
> > F-720-859-4110
> > wk email pruegg <@t> ihctech.net
> > web site www.ihctech.net <http://www.ihctech.net/>
> >
> >
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> >
> >
> > ------------------------------
> >
> > Message: 3
> > Date: Tue, 02 May 2006 10:31:31 -0700
> > From: Patti Loykasek <ploykasek <@t> phenopath.com>
> > Subject: [Histonet] Autostainer tubes
> > To: histonet <histonet <@t> pathology.swmed.edu>
> > Message-ID: <C07CE583.C181%ploykasek <@t> phenopath.com>
> > Content-Type: text/plain; charset="US-ASCII"
> >
> > Opps - didn't put in there a supplier other than
> > Dako. We had a backorder problem with Dako on these.
> > Actually turned out to be a glitch in their system,
> > so all is well. Thanks for all of the good info,
> > though. Histotechs are a nice bunch of people!
> >
> >
> > Patti Loykasek BS, HTL, QIHC
> > PhenoPath Laboratories
> > Seattle, WA
> >
> >
> >
> >
> === message truncated ===
>
>
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--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"Does a conceited cowboy, only ride on pompous grass?.