[Histonet] CD10

Lori Richey lrichey <@t> u.washington.edu
Thu Mar 30 11:29:07 CST 2006

We use Novocastro CD10 at 1:50 diluted in Monet Blue.  Pre treatment is 
15 min microwave in EDTA pH 8,  biotinylated secondary, with Elite 
(Vecta Stain ABC kit)

Theresa Rohr wrote:

>I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval.
>Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. 
>Does anyone out there agree/disagree??? 
>At what dilution???? 
>What I already researched looks like this is not a cheap antibody in terms of dillution. 
>I would appreciate any help.
>Thanks so much,
>Theresa Rohr, Nyack Hospital
>rohrt <@t> nyackhospital.org
>Theresa Rohr, BA, HT(ASCP)
>Section Head, Histology
>Nyack Hospital
>160 North Midland Avenue
>Nyack, New York 10960
>phone 845-348-2276
>fax 845-348-8430
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