[Histonet] CD10
Patti Loykasek
ploykasek <@t> phenopath.com
Wed Mar 29 14:49:46 CST 2006
Hi Theresa. We use the Novocastro antibody at 1:100 with a heat citrate
pretreatment, and Env+ detection done on the Dako Autostainer. For this
antibody our pretreatment is done in a microwave pressure cooker, perhaps
that is why our titer is more dilute than some others have reported. It is a
nice antibody, but not cheap.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
> Hello,
>
> I was wondering if anyone has any suggestions regarding CD10. I have the Dako
> Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval.
>
> Since Dako only has an antibody for use on Frozen sections, I am searching for
> one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a
> great antibody.
>
> Does anyone out there agree/disagree???
> At what dilution????
>
> What I already researched looks like this is not a cheap antibody in terms of
> dillution.
>
> I would appreciate any help.
>
> Thanks so much,
> Theresa Rohr, Nyack Hospital
> rohrt <@t> nyackhospital.org
>
> Theresa Rohr, BA, HT(ASCP)
> Section Head, Histology
> Nyack Hospital
> 160 North Midland Avenue
> Nyack, New York 10960
> phone 845-348-2276
> fax 845-348-8430
>
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