[Histonet] RE: Double staining

[Orr, Rebecca]

Anna,
I am very interested in this.
I am not a chemist, so I would welcome more educated predictions.
But theoretically, this secondary cocktail MIGHT work. My exposure to
this is very limited and is based on my experience with the Biocare
doublestain secondary cocktails. 
You would need to get more information on the actual chemistry of the
secondary components...would the flourophore "smother" out the other
secondary?  I would question how the biotinylated component would cross
react with the flourophore...I have no direct experience to this.
Then there's the dilution of each when they are placed together...
I see the main question is how well the flourophore can cohabitate with
your   biotinylated secondary

Have you tried each stain separately?
Then doing the double stain the "traditional" way?

If you could get a base line, that might help to see what happens when
you start making the voodoo brew.

Let me know how this goes!

 Becky Orr CLA,HT(ASCP)
IHC Lead 
Evanston Northwestern Healthcare
847-570-2771