[Histonet] lipofuscin detection

Kemlo Rogerson kemlo.rogerson <@t> waht.swest.nhs.uk
Thu Jun 29 02:39:20 CDT 2006


It fluoresces brown under ultraviolet light but as with the melanins, there
are many lipofuchsins too. It (lipofuchsin) can be extracted by acetone or
staining prevented by methylation with 0.1N HCL in methanol for 4 to 6 hrs
at 60 degrees C (saponification restores staining, apparently).

Kemlo Rogerson
Pathology Manager
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