[Histonet] lipofuscin detection

Kemlo Rogerson kemlo.rogerson <@t> waht.swest.nhs.uk
Thu Jun 29 02:39:20 CDT 2006

It fluoresces brown under ultraviolet light but as with the melanins, there
are many lipofuchsins too. It (lipofuchsin) can be extracted by acetone or
staining prevented by methylation with 0.1N HCL in methanol for 4 to 6 hrs
at 60 degrees C (saponification restores staining, apparently).

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
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there is a light within. --Elizabeth Kubler-Ross 

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