AW: [Histonet] Some questions about mucins

Gudrun Lang gu.lang <@t>
Thu Jun 29 08:31:18 CDT 2006

 The basic alcianblue binds to anionic charged sides of sulfated and
carboxylated carbohydrates (acid mucins). This works at  pH 2. At lower pH
only sulfated carbohydrates are stained, because the anionic charge of the
others is repressed. 


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Muskett
Gesendet: Donnerstag, 29. Juni 2006 09:38
An: histonet <@t>
Betreff: [Histonet] Some questions about mucins

Dear All
I have been looking through some books and papers prior to giving a tutorial
to my colleagues about carbohydrates and mucins. 2 questions
I seem to recall in the dim and distant past this term was used for Schiff's
reagent. Is this term still applicable?
Alcian Blue mechanism
I am aware that the binding of alcian blue and acid mucins is permanent but
does anyone know the exact mechanism?
Thanks in advance

David Muskett
Chief Biomedical Scientist, Histology
Royal Liverpool Children's NHS Trust
Eaton Road
L12 2AP 
Internal telephone x3615
Telephone (0151) 293 3656
Fax (0151) 293 3617


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