[Histonet] Some questions about mucins

Kemlo Rogerson kemlo.rogerson <@t> waht.swest.nhs.uk
Thu Jun 29 03:05:00 CDT 2006


Alician blues are a family of polyvalent basic dyes with at least two and as
many as four isothiouronium groups per phthalocyanin ring. It works via salt
linkage with polyanions (heparain, DNA and hyaluronate) to give insoluble
precipitates.

(Lillie)

 

Increasing concentrations of electrolytes in the staining solution results
in increased combination of Alcian blue molecules.

 

So it reacts by salt linkages and can be enhanced by increasing electrolyte
concentration (NaCl, KCl, LiBr, mgCl, etc)

 

Kemlo Rogerson

Pathology Manager

Ext  3311

DD   01934 647057

Mob 07749 754194

 

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