[Histonet] Some questions about mucins
Kemlo Rogerson
kemlo.rogerson <@t> waht.swest.nhs.uk
Thu Jun 29 03:05:00 CDT 2006
Alician blues are a family of polyvalent basic dyes with at least two and as
many as four isothiouronium groups per phthalocyanin ring. It works via salt
linkage with polyanions (heparain, DNA and hyaluronate) to give insoluble
precipitates.
(Lillie)
Increasing concentrations of electrolytes in the staining solution results
in increased combination of Alcian blue molecules.
So it reacts by salt linkages and can be enhanced by increasing electrolyte
concentration (NaCl, KCl, LiBr, mgCl, etc)
Kemlo Rogerson
Pathology Manager
Ext 3311
DD 01934 647057
Mob 07749 754194
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