[Histonet] threshhold for antibody binding

[Smith, Allen]

We have an antibody that binds well at a 1:75 dilution, but does not appear
to bind at all when the dilution is 1:85.  Has anyone noticed a similar
phenomenon: a sharp dilution threshhold below which the antibody works well
and above which it does not work at all?

 

Allen A. Smith, Ph.D.

Professor of Anatomy

Barry University School of Graduate Medical Sciences

    Podiatric Medicine and Surgery

Miami Shores, Florida  33161

 

           
                       
                
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