[Histonet] threshhold for antibody binding

[Rene J Buesa]

Allen:
  I had never experienced such a shart "stain-to-not at all" gradient. When diluted below the optimal ratio what I always found was weak staining, but not "no bind at all".
  I do not know why this could happen.
  René J.

"Smith, Allen" <asmith <@t> mail.barry.edu> wrote:
  We have an antibody that binds well at a 1:75 dilution, but does not appear
to bind at all when the dilution is 1:85. Has anyone noticed a similar
phenomenon: a sharp dilution threshhold below which the antibody works well
and above which it does not work at all?



Allen A. Smith, Ph.D.

Professor of Anatomy

Barry University School of Graduate Medical Sciences

Podiatric Medicine and Surgery

Miami Shores, Florida 33161






The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu) 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




 		
---------------------------------
Do you Yahoo!?
 Next-gen email? Have it all with the  all-new Yahoo! Mail Beta.