[Histonet] Marking specimens through decalcification

[Monfils, Paul]

I use black India ink for marking tissues, available from any art supply or
stationery store.  It stays on the surface of the tissue (doesn't tend to
penetrate tissue to any extent), and withstands just about any chemical
treatment (the colorant is carbon particles - pretty inert).

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Pixley, Sarah (pixleysk)
> Sent: 	Friday, June 23, 2006 5:22 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] Marking specimens through decalcification
> 
>  
> Dear Histonet:
> I will be putting rodent nose tissues in 10% formic acid to decalcify
> (after perfusion fixation in 4% paraformaldehyde). I need to mark the
> tissues to keep track of them. What can I use that will withstand the
> several days in formic acid? Each nose will be in a separate Shandon
> biopsy bag.
> 
> Thanks
> Sarah Pixley
> Univ. Cincinnati
> 
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