[Histonet] thawing and re-freezing cryosections

[Hofecker, Jennifer L]

Danielle,
What do you think you might have to do on these slides later?  Are these just going to be kept "just in case" or do you have definte plans for them in the future?  I may have some ideas depending on what will happen to them in the future.
 
Have a great Friday!
 
Jennifer Hofecker, HT (ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
(615) 343-0083
(615) 343-7089 fax

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Danielle Crippen
Sent: Thu 6/22/2006 6:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] thawing and re-freezing cryosections



Dearest Histo-experts,

I have an investigator here who has injected mouse brains with a GFP construct.  They've been perfused with saline and PFA, cryoprotected, and cryosectioned.  But the investigator wants us only to perform IHC on those sections which contain GFP.  In order to determine this, we'll have to air dry the slides.  But I'm wondering how to store those slides which have been air dried but do not contain the GFP.  Can they be stored like paraffin sections at this point (in boxes at RT)...can they be put back at -80?

Any/all advice is VERY welcome!!

Cheers,

Danielle Crippen



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