[Histonet] Superfrost plus slides losing sections

[Mr Andrew Tuck]

Hi everybody

I've had a problem with losing cryostat P0 mouse brain sections from
superfrost plus slides during H&E staining. They are coming off in the
xylene stage of the washes. The sections are allowed to dry for 24hrs in
a laminar flow cabinet before washing. I did use an old box of slides (~
12 months old) for these sections, so that may have something to do with
it. I am re-testing using new slides. I thought these slides were more
"sticky" than anything else. 

Has anybody had this problem ?
Is there a way to prepare the slides to prevent this from occuring ?

Any feedback would be appreciated.

Thanks

  
Andrew Tuck
Research Assistant
Queensland Centre for Schizophrenia Research
School of Biomedical Sciences 
University of Queensland
Australia
Tel: 07 3346 2368