[Histonet] thawing and re-freezing cryosections

[Danielle Crippen]

Dearest Histo-experts,

I have an investigator here who has injected mouse brains with a GFP construct.  They've been perfused with saline and PFA, cryoprotected, and cryosectioned.  But the investigator wants us only to perform IHC on those sections which contain GFP.  In order to determine this, we'll have to air dry the slides.  But I'm wondering how to store those slides which have been air dried but do not contain the GFP.  Can they be stored like paraffin sections at this point (in boxes at RT)...can they be put back at -80?

Any/all advice is VERY welcome!!

Cheers,

Danielle Crippen