[Histonet] brain tissue sections

[Patsy Ruegg]

We are having a terrible time with our ffpe 4 micron rat brain tissue
sections falling off the slides, we are using silane coated slides, air
drying on edge on a small fan, then heating on a slide warmer flat at 55dc
for 1 hour to overnight.  Please help.  The sections are falling off during
deparaffinizing thru xylene and alcohols, haven't even gotten to HIER and
IHC yet.
thanks for your help,
Patsy
 
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/> 
 

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