[Histonet] brain tissue sections

[Favara, Cynthia (NIH/NIAID) [E]]

Patsy,

Has anything changed with fixation or processing? You most likely have
thought of this but it is the first thing that came to mind. 

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net] 
Sent: Thursday, June 08, 2006 11:04 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] brain tissue sections

We are having a terrible time with our ffpe 4 micron rat brain tissue
sections falling off the slides, we are using silane coated slides, air
drying on edge on a small fan, then heating on a slide warmer flat at
55dc
for 1 hour to overnight.  Please help.  The sections are falling off
during
deparaffinizing thru xylene and alcohols, haven't even gotten to HIER
and
IHC yet.
thanks for your help,
Patsy
 
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/> 
 

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