[Histonet] RE: Computer software to decrease background

[nienhuis <@t> ucla.edu]

There is also software that reduces background scatter from florescence
that radiates 360 degrees from the object.
Autoquant AutoDeblur has been used to good effect even on confocal
images. $$$!

Bob Nienhuis

Quoting "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>:

>
>   Dear all,
>
>   There  is  software  (+  hardware) indeed to separate autofluorescence
>   from  real  fluorescence. Spectral imaging is the way to unmix signals
>   based  on  their  spectral  characteristics.  Autofluorescence  has  a
>   slightly  different  spectral  characteristic than "real" fluorescence
>   and can  therefore  be  nicely  separated  from each other. At the CRI
>   website  ([1]http://www.cri-inc.com/products/nuance.asp) you should be
>   able  to  find  some good examples of it. Sorry folks I work with that
>   great  system  now  for  a  couple  of months for both brightfield and
>   fluorescence, but I never considered it as being fraud.......
>
>   NB: you will see it isn't "fraud" in NSH workshop #2: "Immunoenzymatic
>   multiple  staining methods - A practical overview and possibilities of
>   multi-color  imaging  by  spectral analysis" by Chris van der Loos and
>   Richard Levenson. (Saturday September 9th, whole day)
>
>   Chris van der Loos, PhD
>   Dept. of Pathology
>   Academic Medical Center M2-230
>   Meibergdreef 9
>   NL-1105 AZ Amsterdam
>   The Netherlands
>
>   Date: Thu, 08 Jun 2006 09:27:30 -0600
>   From: Gayle Callis <gcallis <@t> montana.edu>
>   Subject: Re: [Histonet] Computer software to decrease background
>   staining in Immunoflouresence staining
>   To: Geoff McAuliffe <mcauliff <@t> umdnj.edu>,
>   Histonet <@t> lists.utsouthwestern.edu,
>   [2]Histonet <@t> lists.utsouthwestern.edu
>   Geoff is absolutely correct!!!
>   Gayle CAllis
>   At 07:24 AM 6/8/2006, you wrote:
>   >If  you  improve the quality of your immunostaining you won't need to
>   do it
>   >with  at  computer.  Removing/reducing   background  with  a computer
>   program
>   >could   be   considered   fraud.   Most   journals  can  detect  such
>   manipulations and
>   >will reject your work on that basis alone.
>   >
>   >Geoff
>   >
>   >sohail ejaz wrote:
>   >
>   >>Hi every body
>   >>
>   >>   Is  there  any  one who can recommend me any Computer software to
>   decrease
>   >> backgroun! d stainin
>
> References
>
>   Visible links
>   1. http://www.cri-inc.com/products/nuance.asp
>   2. mailto:Histonet <@t> lists.utsouthwestern.edu
>
>   Hidden links:
>   3. http://www.cri-inc.com/
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