[Histonet] reply: Ncrosis detection

[PKamalavenkatesh <@t> wockhardtin.com]

Necrosis is currently used to indicate non apoptotic accidental form of
cell death. But please understand that cell death and necrosis are two
different entities. For example if rat liver cells are subjected to
ischemia, hydrolysis and denaturation of cellular proteins started at 30
minutes and cells reached the point of no return (this is actually the cell
death) at 2.0-2.5 hrs. This point of no return is characterized by massive
caspase activation, loss of mitochondrial transmembrane potential and
exposure of phosphatidylserine groups, which make the cells to be
identified by the phagocytic cells, and aids in phagocytosis (I can tell
EAT ME signal). Now histological identification will come only at 12-24
hrs. That means cells die long before any necrotic changes seen under light
microscope. So cell death and necrosis are two different entities and
should not be used as synonyms. If you have any queries, please get back to
me. I have some superb references dealing with this always controversial
topic.


REGARDS

Dr.P.Kamalavenkatesh

Discovery- Biology

Pre clinical Safety Assessment

Wockhardt Research Center, Aurangabad, India.

E.mail : Pkamalavenkatesh <@t> wockhardtin.com


Please Visit our New Corporate Web Site www.wockhardt.com
--------------------- Disclaimer ------------------------------------------------------------------------------
Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies 
and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, 
and may contain information that is privileged, confidential or exempt from disclosure under applicable law. 
If you are not the intended recipient or it appears that this mail has been forwarded to you without proper 
authority, you are notified that any use or dissemination of this information in any manner is strictly 
prohibited. In such cases, please delete this mail from your records.
---------------------------------------------------------------------------------------------------------------