[Histonet] Leica microtome

[Charles Scouten]

Usually, when we hear about such things, the answer is the blade angle
is too shallow, or the pedestal has wobble for some reason.  Grasp the
tissue pedastal with your hand and try to wobble it.  If you can, that
is the problem. Has the blade angle changed, on purpose or by accident?
Also try to wiggle the blade to see if it is loose, but try to do that
without losing a hand.  


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dr. F.
Tryka
Sent: Friday, July 14, 2006 2:36 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica microtome

We are seeking advice.  Our Leica 2125 RM microtome has been functioning
well until it suddenly started cutting thick thin sections.  
We have tried to align it, etc and it still is not functioning properly.

 
The block face looks smooth, but it will cut several slices toward one
side, then on the next pass it cuts off a potato chip slice.  Any
suggestions would be appreciated.  Franci Tryka


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