[Histonet] safranin o staining-proteoglycans-thanks

[PKamalavenkatesh <@t> wockhardtin.com]

Dear Histonetters,
                             This  is with regard to safranin-O & Toluidine
blue  staining of rat bone cartilage. At last I got a perfect and beautiful
staining  of  cartilage.  The procedure is as given by Gayle Callis and the
fixative  and  decalcification  fluid  are  10 % NBF & buffered formic acid
respectively. As our experts in histonet pointed out, that the real culprit
was  EDTA  used  as  decalcification  fluid.  Thanks to all for their sound
suggestions and prompt responses.

REGARDS
Dr.P.Kamalavenkatesh
Drug Discovery- Biology
Pre clinical Safety Assessment
Wockhardt Research Center, Aurangabad, India.
E.mail : Pkamalavenkatesh <@t> wockhardtin.com


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