[Histonet] Alpha Smooth Muscle Actin -IHC

[Wulan Anggrahini]

Is there anyone doing IHC for alpha SMA (mouse anti-aSMA Sigma, clone 1A4) 
on mouse cryostat section ? I did on carotid artery tissue and I used 1/100 
dilution overnight and secondary antibody is Histofine Max-PO (Mouse) in 
1/200 dilution overnight.

Problem is keep having background with low signal/background ratio. Anyone 
has any tips ? I keep trying for 2 months already and now it starts to make 
me frustating. Thanks before

Wulan Anggrahini
Japan