[Histonet] Slide storage

Morken, Tim - Labvision tpmorken <@t> labvision.com
Thu Jan 19 13:24:56 CST 2006

Rebecca, you might be interested in this study on tissue array longevity.

Lab Invest. 2004 Aug;84(8):1071-8. 

Long-term preservation of antigenicity on tissue microarrays.

DiVito KA, Charette LA, Rimm DL, Camp RL.

Department of Pathology, Yale University, New Haven, CT, USA.

Tissue microarrays have facilitated the evaluation of large cohort studies;
however, there is little data on the best method for preserving sections
once they are cut. We assessed three methods of storing precut breast cancer
microarray slides: paraffin coating and storage in a nitrogen desiccator,
either alone or in combination. We tested the durability of three antigens,
cytokeratin, estrogen receptor, and Ki-67 on microarrays stored under these
conditions for 3 months at room temperature. Staining was assessed with both
manual scoring using traditional brown stain (0-3+) as well as automated
scoring using fluorescently stained sections. Staining intensity was
compared to that obtained from freshly cut slides. Slides stored under
ambient conditions (room temperature and air) for 3 months exhibited marked
degradation of all target antigens, in some cases resulting in slides that
were virtually unreadable. We found that combined paraffin coating and
nitrogen storage resulted in the best preservation of antigenicity, with
retention of 72-99% of the antigenicity of a freshly cut slide, depending
upon the marker and detection system used. The use of either paraffin
coating or nitrogen storage alone protected slides to a lesser degree.

PMID: 15195116 [PubMed - indexed for MEDLINE] 

Tim Morken
Lab Vision - Neomarkers

Free webhosting for US State Histotechnology Societies:

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, January 19, 2006 10:56 AM
To: Orr, Rebecca; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Slide storage

  Try to isolate the slides as much from air as you can. Inside a plastic
box (those that
  can contain 25 slides) sealed in a pouch with as less air as possible and
in a
  refrigerator will be the best option.
  In a paper I wrote (JOH 28(2):89-97, 2005) I was able to demonstrate that
"normal" storage of slides in the lab. environment for more than 6 weeks
reduces epitope reactivity in a statistically significant way.
  René J.

"Orr, Rebecca" <ROrr <@t> enh.org> wrote:
  Hello everyone,

I need to store unstained slides for what might be 6 months to a year. These
slides may be used for some IHC staining. I do not know what anibodies, but
I would imagine they are not the ones used in a routine clinical lab

I have some options, for storage, please give opinions.

Store the slides in a slide mailer, then wrap in foil and place in -80

Or one of my pathologists talked about coating each unstained slide in a
layer of paraffin. Has anyone done this technique?

I would assume that I just cut, dry, then melt the slide, then dip the slide
in paraffin?

Many thanks for your help.


Becky Orr CLA,HT(ASCP)

IHC Lead 

Evanston Northwestern Healthcare


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