[Histonet] Eosin too pink
Marshall Terry Dr,
Terry.Marshall <@t> rothgen.nhs.uk
Fri Jan 13 11:54:18 CST 2006
Wouldn't we all *love* to see just what is considered too pink:-)
An old pathologist friend of mine used to put a pinch of bicarb in the eosin when the techs were not looking.
He had a thing of not liking it too pink. For me, I can't have it too pink, as long as the nuclei are blue.
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Rotherham General Hospital
terry.marshall <@t> rothgen.nhs.uk
From: Parker, Helayne [mailto:HParker <@t> Skaggs.Net]
Sent: 13 January 2006 16:42
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin too pink
We are currently doing our H & E stain by hand and using the
Richard-Allan 7211 Hematoxylin and their Eosin Y. Our pathologist is
still complaining of slides being TOO PINK. I have cut the eosin step
down to just 5 dips from 15 dips, and still too pink. The Eosin is very
This is our protocol:
Hydrate to water like normal (thru Clear Rite 3, 100% and 95% Flex)
Hematoxylin- 2 mins
Rinsing in water until the water runs clear
Clarifier II- 30 dips
running water -30 dips
Richard Allan Bluing - 30 dips
running water - 30 dips
95% Flex - 10 dips
Eosin-Y- 5 quick dips
Thru 95%, 100% etc to xylene
Any suggestions on a possible method change or a more mild Eosin.
Complaint seems to solely "TOO PINK". I have done some experimenting w/
cutting the eosin w/ 80% Flex- and it does calm it down a bit but, the
Hemo looks more purply then blue, almost periwinkle so not sure if that
will be better or not- we still need to have him review the test slides.
Any suggestions ?
Histology Section Head
Skaggs Community Health Center
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