Fw: [Histonet] PGP 9.5

[Ian Montgomery]

Peggy,
            PGP9.5 (monoclonal) from Novocastra works a treat. Whole mounts,
cryostat, FFPE, with any demonstration technique works. Used it on various
species, including man, with success.
Ian.


----- Original Message ----- 
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 22, 2006 12:32 AM
Subject: [Histonet] PGP 9.5


> Anyone have experience with PGP 9.5 (Protein Gene Product)? It
demonstrates
> nerve fibers. One of our pathologists wants to use it on skin biopsies for
> diagnosis of neuropathies.
>
> We would prefer peroxidase for paraffin sections, rather than FITC or FS.
> Just wondering if people are having better luck with monoclonals or
> polyclonals, and if there is a difference in animal species for the
primary
> (we'll be doing this on human skin).
>
> Also - all the papers we find mention thick sections and using a confocal
> microscope. Anyone doing PGP on 5 um sections and/or with a regular light
> microscope?
>
> One of my students is doing this as her research project. She's done a lot
> of reading on it, and most papers don't discuss the actual procedure or
the
> type of antibody, etc. They are on the research aspect, or the diagnostic
> aspect, not on what histotechs need to do the procedures. Those articles
> that do mention techniques have very different methods (of course), and
are
> missing important information needed by histotechs.
>
> We would appreciate a couple of little hints of what works for "real life
> histotechs", to get us started in the right direction for use in a routine
> clinical immunohistochemistry lab.
>
> Feel free to contact me directly. Vendors included. I'll summarize and
relay
> the info back to Histonet.
>
> Thanks in advance.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
>
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