[Histonet] Ehrlich Biondi Heidenhain stain

[Massimo]

Hi all,

does someone know the protocol for staining with the “Ehrlich Biondi Heidenhain” method?
Particularly how long has the specimen to stay in the staining solution?
I have noticed, in previous, tests that is quit difficult to dye the nucleus with the methyl green contained in the solution.
I am processing thin sections of mouse liver included in wax and fixed with Bouin liquid.
I also wonder why the sections are crunched after cutting. There is a solution at this problem?

Thanks in advance.
Best Regards,

Massimo

Università degli Studi di Pisa
Facoltà di Ingegneria Chimica
Italy