[Histonet] Attention to those doing immunofluorescent staining, looking for mounting media

[Gayle Callis]

Dear Histonetters doing immunofluorescence staining,

Microscopy Today 14(1):34-39, January, 2006 just published Mounting Media 
and Antifade Reagents, Collins TJ

This publication is a wonderful comparison of 10 different mounting medias 
for IFA work along with excellent discussion.  I strongly recommend 
accessing and keeping a copy of this article around.  It was not an 
endorsement, but certainly educational and useful.

The only thing he did not include was using diluted (in it's own solvent) 
permanent mounting media (toluene or xylene based) instead of nail polish 
containing isopropyl alcohol as a sealant with nail polish (cited in 
literature)  known to ruin GFP and cause it to not fluoresce.

My thanks to Mr. Collins!!

Enjoy





Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)