[Histonet] microwave processing on a budget...
Anthony Reilly
Tony_Reilly <@t> health.qld.gov.au
Tue Dec 19 00:51:38 CST 2006
Hi Debbie
I will be interested to hear other subscriber's comments with regard
to
microwave processing of skin. In our lab we do not have a microwave
processor but we do microwave fix on a regular basis. Having
said that we always resist microwave fixing skin as the process
damages
the skin tissue giving it that "overcooked" appearance which occurs
when
tissue has been exposed to too much heat during processing. This is a
genuine artifact as we have found in a whole container of breast
tissue
which has been microwave fixed only the nipple will be damaged.
With regard to your second hand processor, it is a bit like buying a
second
hand lawn mower, you get what you paid for. If it is a shandon from
circa
late 1980s early 1990s they were notoriously flawed, particularly the
heating bands for the retort.( Current models are fine) A new modern
standard processor will give you good morphology on small skins on a
2-4 hour processing run and larger skins dependent on the amount of
fat
present will process well on 9-12 hour cycles.
While microwave processors have their place in Histology for the high
throughput laboratories that are running 18-24 hours per day, if your
lab is working predominately day shifts only, I do not believe the
advantages of the microwave processors will be achieved and therefore
the extra cost of purchase is not warranted. As well as this most
microwave processors require the user to purchase the manufacturer's
"secret" proprietry solution/s creating further ongoing costs.
regards
Tony Reilly
Chief Scientist
Anatomical Pathology
QHPS-Prince Charles Hospital
Rode Rd Chermside Q 4032
Australia
Ph: 07 3139 4543
Fax: 07 3193 4546
tony_reilly <@t> health.qld.gov.au
>>> Debbie Keith <debbiekeith <@t> cox.net> 12/19/06 3:46 am >>>
Hey all...
i have a few questions about microwave processing skin biopsies
utilizing a
microprocessor. i've looked into protocols and equipment but figured
this
would be the best/quickest sources of trial/error stories.
i want to get a used lab microwave but want to hear good/bad stories
from
the folks in the trenches. :) marketers can sell me ANYTHING!! i
might be
the ONLY person that bought the magic bullet food processor AND the
ginsu
knife. (for the record, they are NOT as sharp after cutting through a
tin
can. fyi.)
i purchased a used shandon hypercenter from a surplus lab equipment
place... and it's been such a NIGHTMARE. with enclosed processors
there
are so many possible issues. gaskets/circuit boards/cable ports....
i'm
starting to think micro-processing would keep the biomedical-nightmares
to
a minimum. have i inhaled too many fumes or could i be onto something?
i REALLY don't want to resort to an old carousel processor.
the specimens will be limited to small shave/punch skin biopsies and
the
runs will never be more than 20 blocks. i would love to hear from
anyone
that has done this with good results. i'd like to hear the "avoid THIS
at
all costs...." stories as well. :)
histonette is such a wonderful resource! you guys are AWESOME!
debbie
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